Virucidal Efficacy of Pre-Saturated Towelettes for Hard Surface Disinfection
Purpose of Test
This test method is used to evaluate the efficacy of pre-saturated disinfectant towelettes for registration as a virucide for hard, non-porous surfaces with regulatory agencies such as the U.S. EPA and Health Canada. The test procedure is to simulate the way in which the product is intended to be used. This test method may be performed using a wide variety of human and veterinary viruses including human Hepatitis B virus utilizing duck Hepatitis B virus as the surrogate, human Hepatitis C virus utilizing Bovine Viral Diarrhea virus as the surrogate, and human Norovirus utilizing Feline Calicivirus as the surrogate.
Summary of Test
A glass Petri dish (“carrier”) is inoculated with a representative test virus and the virus is dried onto the carrier. The carrier is wiped with the towelette and the carrier is held for the requested exposure time at the requested exposure temperature. Following exposure, the contents of the carrier are reconstituted and neutralized. Serial dilutions of the neutralized test substance are then performed and the dilutions are assayed for viral infectivity by an assay method specific for the test virus. Appropriate virus, test substance cytotoxicity, and neutralization controls are run concurrently. Typically, most regulatory agencies require complete inactivation of the virus in all dilutions assayed.