Test Method for the Continuous Reduction of Organisms on Treated Surfaces

Purpose of Test

The continuous reduction of organisms on treated surfaces test is a carrier-based test method used to evaluate treated articles (such as antimicrobial metal alloys) for the continuous reduction of pathogens for public health claims. These products are typically registered with the U.S. EPA.

Summary of Test

In this method, a series of treated surfaces are continually inoculated with the test organism at prescribed intervals over the course of 24 hours. Over a variety of time points between 2 hours and 21 hours of exposure, the inoculated surfaces are neutralized and quantitatively assayed for survivors. The resulting plates are incubated, the number of survivors is enumerated and a percent reduction is determined as compared to an untreated population control. In order to successfully demonstrate continuous reduction efficacy, the product must demonstrate at least a 90% reduction at each time point over the course of 24 hours. Typical test organisms include Staphylococcus aureus and Enterobacter aerogenes. Additional pathogens of clinical, occupational or household relevance including but not limited to: Pseudomonas aeruginosa, Escherichia coli O157:H7 and Methicillin Resistance Staphylococcus aureus – MRSA are often tested as well.