Test Method for Determining the Residual Self-Sanitizing Activity of Treated Surfaces

Purpose of Test

The Test Method for Determining the Residual Self-Sanitizing Activity of Antimicrobial Surfaces is a carrier-based test method used to evaluate treated articles (such as antimicrobial metal alloys) for residual self-sanitizing efficacy of pathogens for public health claims. These products are typically registered with the U.S. EPA.

Summary of Test

In this method, a set of treated surfaces undergo a series of physical wear procedures followed by systematic low-level inoculation of test organism to simulate routine use and contamination of the treated surface. After completion of the wear cycles, the treated surfaces are inoculated with the test organism to evaluate the residual sanitizing efficacy of the surface and the survivors are quantitatively assayed. The resulting plates are incubated, the number of survivors is enumerated and a percent reduction is determined as compared to an untreated population control. In order to successfully demonstrate continuous reduction efficacy, the product must demonstrate at least a 99.9% reduction typically within 2 hours. Typical test organisms include Staphylococcus aureus and Enterobacter aerogenes. Additional pathogens of clinical, occupational or household relevance including but not limited to: Pseudomonas aeruginosa, Escherichia coli O157:H7 and Methicillin Resistance Staphylococcus aureus – MRSA are often tested as well.