Test Method for Determining the Efficacy of Antimicrobial Surfaces as Sanitizers

Purpose of Test

The Test Method for Determining the Efficacy of Antimicrobial Surfaces as Sanitizers is a carrier-based test method used to evaluate treated articles (such as antimicrobial metal alloys) for sanitizing efficacy of pathogens for public health claims. These products are typically registered with the U.S. EPA.

Summary of Test

In this method, a set of treated surfaces are inoculated with the test organism and are dried. Following drying, the test organism is allowed to expose to the treated surface. Following exposure, the surfaces are neutralized and quantitatively assayed for survivors. The resulting plates are incubated, the number of survivors is enumerated and a percent reduction is determined as compared to an untreated population control. In order to successfully demonstrate continuous reduction efficacy, the product must demonstrate at least a 99.9% reduction typically within 2 hours. Typical test organisms include Staphylococcus aureus and Enterobacter aerogenes. Additional pathogens of clinical, occupational or household relevance including but not limited to: Pseudomonas aeruginosa, Escherichia coli O157:H7 and Methicillin Resistance Staphylococcus aureus – MRSA are often tested as well.