Single Tube Method for Determining the Efficacy of Disinfectants Against Bacterial Biofilm (Pseudomonas aeruginosa and Staphylococcus aureus)

Posted on: December 18, 2017

Purpose of Test

The Single Tube Method for Determining the Efficacy of Disinfectants against Bacterial Biofilm test method is used to evaluate liquid disinfectants against biofilm bacteria (Pseudomonas aeruginosa and Staphylococcus aureus) in a closed, single tube system.

Summary of Test

A biofilm is grown onto a series of coupon “carriers” and treated with a sample of product and allowed to expose. Following exposure, the carriers are neutralized and assayed for surviving bacteria. The resulting plates are incubated, enumerated, and the Log10 reduction is determined as compared to the population control. For biofilm claims, a 6 Log10 reduction must be observed. Currently draft guidance has been published to follow U.S. EPA BEAD SOPs.