OECD Quantitative Method for Testing Antimicrobial Products Against Spores of Clostridium difficile (ATCC 43598) on Inanimate, Hard, Non-porous Surfaces

Posted on: December 18, 2017

Purpose of Test

The OECD Quantitative Method for Testing Antimicrobial Products Against Spores of Clostridium difficile (ATCC 43598) on Inanimate, Hard, Non-porous Surfaces is a carrier-based test method designed to evaluate chemicals for effectiveness against Clostridium difficile spores. The method has been implemented as a guidance document which follows U.S. EPA BEAD SOPs and is recognized by the U.S. EPA.

Summary of Test

In this method, a series of stainless steel disks are inoculated with test organism. The carriers are desiccated and subsequently treated with a sample of the disinfectant. After exposure, the carriers are neutralized and quantitatively assayed for surviving test organism. The resulting plates are incubated, the number of survivors is enumerated and a Log10 reduction is determined as compared to the population control. In order to successfully demonstrate disinfection efficacy, the product must demonstrate a 6 Log10 reduction of Clostridium difficile spores. In addition, the disinfectant must have verification testing performed resulting in a 6 Log10 reduction of Clostridium difficile spores.