JIS Z 2801 / ISO 22196 (modification) – Japanese Industrial Standard Test for Determining Antiviral Activity
Posted on: December 5, 2014
Purpose of Test
The JIS Z 2801 and ISO 22196 standard test methods modified for viruses are used to evaluate the efficacy of antiviral plastic, metal, and ceramic-based materials. This method is generally used to substantiate non-public health related antibacterial claims. Typical test organisms utilized in this method are Hepatitis A virus, Norovirus surrogate, Rotavirus and additional pathogens of clinical, occupational, or household relevance.
Summary of Test
In these methods, treated test samples and untreated control samples are inoculated with the test virus. The test virus is held in place under a flexible coverslip and the samples are allowed to expose at the requested exposure temperature and the requested exposure time. Following exposure, the samples are neutralized, serial dilutions are performed of the neutralized solution and the dilutions are then assayed for viral infectivity by an assay method specific for the test virus. Appropriate virus, test substance cytotoxicity, and neutralization controls are run concurrently. The percent and log reduction in viral infectivity are calculated as compared to the corresponding virus control. No regulated performance criteria currently exist for antiviral claims made using these methods.