JIS Z 2801 / ISO 22196 Japanese Industrial Standard Test for Determining Antimicrobial Activity

Purpose of Test

The JIS Z 2801 and ISO 22196 standard test methods are used to evaluate the efficacy of antibacterial plastics, metals, and ceramics. This method is generally used to substantiate antibacterial properties of treated products for non-public health related claims.

Summary of Test

In these methods, treated test samples and untreated control samples are inoculated with a representative test organism, commonly Staphylococcus aureus and Escherichia coli. The test organism is held in place under a flexible coverslip and the samples are allowed to expose at an elevated temperature, typically for 24 hours. Following exposure, the samples are neutralized and quantitatively assayed for survivors. The resulting plates are incubated, the number of survivors is enumerated and a percent and log10 reduction is determined for the treated test material as compared to the untreated control material. No regulated reduction limits currently exist for general antibacterial claims made using these methods.