EN 14347 Chemical Disinfectants and Antiseptics: Quantitative Test for Basic Sporicidal Activity

Summary of Test

The EN method below are designed to evaluate the bactericidal, fungicidal, yeasticidal, basic sporicidal or mycobactericidal activity of a product used under various conditions. Many methods are suspension based and are used to support general antimicrobial claims while other test methods are carrier-based and are used to support antimicrobial activity of products on surfaces or devices. In either case, the same general principles apply. The test organism is exposed to sample of the product in a manner which simulates the desired claim. Following exposure, the test system is neutralized and quantitatively assayed for survivors. The resulting plates are incubated, enumerated, and a reduction in viability or microbiocidal effect is determined as compared to a population control.

Test Organisms Bacillus subtilis, Bacillus cereus
Purpose of Test Suspension-based study used as a presumptive test to evaluate basic sporicidal activity.
Typical Performance Criteria(Requirements may vary by claim) 4 log reduction in ≤120 minutes