ASTM E2362 / AOAC Method 961.02 (Modification) Pre-Saturated Towelettes for Hard Surface Disinfection Test Method

Purpose of Test

The ASTM E2362 Method for Pre-Saturated Towelettes for Hard Surface Disinfection or a modification of the AOAC 961.02 Germicidal Spray Method may be used to evaluate single-use towelettes for disinfection efficacy. These products are typically registered with regulatory agencies such as the U.S. EPA and Health Canada.

Summary of Test

In this method, a series of glass slides (aka “carriers”) are inoculated with a representative test organism and the carriers are dried. The carriers containing the dried organism film are then sequentially treated with the towelette product and are exposed for a pre-determined exposure time (≤10 minutes). After exposure, the carriers are sequentially transferred to a liquid subculture medium specifically selected to neutralize the test substance active and to recover any surviving test organism. The carriers are incubated and visually examined for the presence or absence of growth. Based on the desired disinfection claim and the requirements of the regulatory agency, the product must demonstrate kill on a pre-determined number of carriers inoculated with test organisms applicable to the claim. Standard disinfection organisms include but are not limited to: Staphylococcus aureus, Salmonella enterica, and Pseudomonas aeruginosa. Additional pathogens of clinical, occupational or household relevance including fungi are often tested.