ASTM E2149 Standard Test Method for Determining the Antimicrobial Activity of Immobilized Antimicrobial Agents

Purpose of Test

The ASTM E2149 Standard Test Method is used to evaluate the antimicrobial activity of surface-bound, non-leaching, antimicrobial-treated specimens under dynamic contact conditions. Materials evaluated by this method may be comprised of fabric, paper, powder or other solid materials. This method is generally used to substantiate antimicrobial properties of treated specimens for non-public health related claims.

Summary of Test

In this method, treated test samples are placed in a laboratory flask containing a dilute suspension of test organism, commonly Escherichia coli. The flask is placed onto a wrist-action shaker and shaken for a desired exposure time, typically 1 hour. Following exposure, a sample of the test organism suspension is removed quantitatively assayed for survivors. The resulting plates are incubated, the number of survivors is enumerated and a percent reduction is determined for the test flask as compared to the untreated control suspension. No regulated reduction limits currently exist for general antimicrobial claims made using this method.