ASTM E1153 Standard Test Method for Efficacy of Sanitizers Recommended for Inanimate Non-Food Contact Surfaces

Purpose of Test

The ASTM E1153 Standard Test Method for Efficacy of Sanitizers Recommended for Inanimate Non-Food Contact Surfaces is a carrier-based test method used to evaluate water soluble powders, liquid, spray or towelette products intended to reduce the number of microorganisms on non-food contact surfaces (e.g. floors, walls and furnishings). These products are typically registered with the U.S. EPA.

Summary of Test

In this method, a series of glass slides (“carriers”) are inoculated with a representative test organism and the carriers are dried. The carriers containing the dried organism film are then sequentially treated with the product in a manner that represents its intended use and are exposed for a pre-determined exposure time. After exposure, the carriers are neutralized and quantitatively assayed for survivors. The resulting plates are incubated, the number of survivors is enumerated and a percent reduction is determined as compared to an untreated population control. In order to successfully demonstrate sanitizing efficacy, the product must demonstrate a 99.9% reduction. Typical test organisms include Staphylococcus aureus and Enterobacter aerogenes or Klebsiella pneumoniae. Additional pathogens of clinical, occupational or household relevance are often tested as well.