ASTM E1052 Standard Test Method for Efficacy of Antimicrobial Agents Against Viruses in Suspension

Purpose of Test

The ASTM E1052 Standard Test Method for Efficacy of Antimicrobial Agents against Viruses is a suspension based method which can be used to determine the efficacy of a variety of water soluble or liquid products against viruses. In addition to serving as an excellent screening tool during the development stages, this method is commonly used to evaluate hand sanitizers as recommended by the U.S. FDA’s Tentative Final Monograph for OTC Healthcare Antiseptic Drug Products. Typical test organisms utilized in this method include Adenovirus, Coronavirus, Influenza viruses, Rhinovirus, Rotavirus and additional pathogens of clinical, occupational or household relevance.

Summary of Test

An aliquot of the test substance is inoculated with the test virus and held for the requested exposure time(s). At each pre-determined exposure time an aliquot is removed, neutralized by serial dilution, and assayed for viral infectivity by an assay method specific for the test virus. Appropriate virus, test substance cytotoxicity, and neutralization controls are run concurrently. The percent and log reduction in viral infectivity are calculated as compared to the corresponding virus control.