AOAC Method 965.12 Tuberculocidal Activity of Disinfectants

Purpose of Test

The AOAC Tuberculocidal Activity of Disinfectants test method is a carrier-based method used to evaluate water soluble powders, liquid products, spray products and single-use towelettes for tuberculocidal efficacy for registration with regulatory agencies such as the U.S. EPA, Health Canada and in the case of high-level disinfectants, the U.S. FDA.

Summary of Test

In this method, a series of porcelain cylinders or glass slides (“carriers”) are inoculated with Mycobacterium bovis – BCG and the carriers are dried. The carriers containing the dried organism film are then sequentially treated with the disinfectant in a manner intended to simulate its intended use and are exposed for a pre-determined exposure time. After exposure, the carriers are sequentially transferred to a series of liquid media specifically selected to neutralize the test substance active and subsequently recover Myocbacterium. The subculture media is incubated and visually examined for the presence or absence of growth. For tuberculocidal claims, all subcultures must demonstrate kill (no growth) of Mycobacterium bovis– BCG.