AOAC Method 961.02 Germicidal Spray Method

Purpose of Test

The AOAC Germicidal Spray test method is a carrier-based method used to evaluate disinfection efficacy of aerosol/pump-based spray products and volatile liquid products for registration with regulatory agencies such as the U.S. EPA and Health Canada.

Summary of Test

In this method, a series of glass slides (“carriers”) are inoculated with a representative test organism and the carriers are dried. The carriers containing the dried organism film are then sequentially treated with the spray product and are exposed for a pre-determined exposure time. After exposure, the carriers are sequentially transferred to a liquid subculture medium specifically selected to neutralize the test substance active and to recover any surviving test organism. The carriers are incubated and visually examined for the presence or absence of growth. Based on the desired disinfection claim and the requirements of the regulatory agency, the product must demonstrate kill on a pre-determined number of carriers inoculated with test organisms applicable to the claim. Standard disinfection organisms include but are not limited to: Staphylococcus aureus, Salmonella enterica, and Pseudomonas aeruginosa. Additional pathogens of clinical, occupational or household relevance including fungi are often tested.