AATCC 100 Residual Self-Sanitizing Activity of Laundry Additives

Purpose of Test

The AATCC 100 in conjunction with the ASTM E2274 or ASTM E2406 test methods is a carrier-based test method used to evaluate the residual self-sanitizing properties of laundry detergents/additives designed for top-loading and front-loading washing machine operations. These products are typically registered with the U.S. EPA.

Summary of Test

In this method, laundry fabric is pre-treated with the product in a simulated laundry operation. The treated fabric is removed and air-dried. Following drying, treated fabric samples and untreated control samples are inoculated with a representative the test organism, commonly Staphylococcus aureus, Klebsiella pneumoniae and Pseudomonas aeruginosa. The test organism is allowed to expose to the test material for a series of time points up to 24 hours. Following each exposure time, the samples are neutralized and quantitatively assayed for survivors. The resulting plates are incubated, the number of survivors is enumerated and a percent and log10 reduction is determined for the treated test material as compared to the untreated control material. For residual self-sanitizer label claims, the U.S. EPA requires that the product demonstrate a 99.9% (3 log10) reduction of the test organism as compared to the untreated control at the desired exposure time.